ECIS® History and principle

ECIS® Technology History and principle

The technology of measuring in real time changes in electrical impedance of a cell layer_ECIS® for Electric Cell-substrat Impedance Sensing_  was invented by Ivar Giaever (Nobel Prize in Physics) and Charles Keese within the Society of General Electrics and then sold by the company Applied Biophysics (which they are the founders and owners)

What is impedance?

Impedance reflects the measurement of the resistance to alternating current. The ECIS® technology measures the impedance (resistance) of a cell layer to the passage of alternating current. This measure, whose reading is done in real time, is a direct reflection of cell behavior.

How to measure cell impedance?

Cells were plated on cell culture dishes wells containing gold electrodes at the bottom. An electric current of very low intensity is then applied. The presence of cell opposes a resistance to the passage of this current (impedance). This resistance is measured and collected in real time.