Robust, Sensitive and Predictive Assays for in vitro toxicity and hepatotoxicity: Impedance-Based Cellular Application for ECIS platform.
The in vitro study of xenobiotic toxicity is a critical phase of any component in the development phase. Although there are dozens of regulatory and non-regulatory tests (OECD guideline), only ECIS platform because of its features can provide a global and predictive datas concerning the in vitro toxicity of the component develop by R&D.It is now possible with a single platform:
- to carry out acute toxicity studies and define the EC50
- to perform chronic toxicity studies
- to establish the kinetic effect of the component
- to study precisely the mixing effect (synergistic effect distinction, additive, antagonist)
- to establish a precise mapping (dynamic mapping) of key moments (passage between two cell states) the components effect: dynamic toxicology
- to identify in some cases the mechanism of action of the tested components using dynamic mapping.
- to carry out medim-throughput screening.
All of these points demonstrate that the platform ECIS® undoubtedly offers significant productivity gain for xenobiotic toxicity studies on liver cells in particular (see publication).
ECIS® platform is the better way to improve the in vitro preclinical research.
Hepatotoxicity is the most common reason for a drug to be withdrawn from the market.
The liver plays a central role in transforming and clearing chemicals and is susceptible to the toxicity from these agents.
Drug-induced liver injury is responsible for 5% of all hospital admissions and 50% of all acute liver failures.
Preclinical studies of hepatotoxicity have a major role in the development of new therapeutic molecules.
The ECIS® impedance platform offers a comprehensive and dynamic approach for in vitro pre-clinical studies hepatotoxicity.
It becomes possible with a single platform:
- to carry out acute toxicity studies and EC50;
- to perform chronic toxicity studies;
- to establish the kinetic of component’s effect;
- to establish whether the toxicity is induced by component or its metabolites (see publication)
- to study the mixing effect (distinction synergistic, additive, antagonist)
- dynamic toxicology to establish a precise mapping of key moments (passage between two cell states) of components effect. (the dynamic mapping is important for the realization of screening (compared curves) or to determine completion time of the end-point.)
- To identify in some cases the mechanism of action of the tested components: dynamic mapping.
- to achieve the medium throughput screening.
High-content screening imaging and real-time cellular impedance monitoring for the assessment of chemical's bio-activation with regards hepatotoxicity Journal Article
Toxicol In Vitro., 29 (7), pp. 1916-1931, 2015.
BK/TD models for analyzing in vitro impedance data on cytotoxicity Journal Article
Toxicol Lett., 235 (2), pp. 96-106, 2015, ( ).